In Vitro Human Megacaryocytopoiesis: Determination of Thrombopoietin Optimal Concentration
Abstract
The study of human megakaryocytopoiesis has been hampered by the relative scarcity of megakaryocytes
in bone marrow (0.05-0.2 % of medullary cells), which has led to the optimization of protocols of in vitro
expansion of precursors from diverse sources (umbilical cord, bone marrow and peripheral blood with or
without previous mobilization). Cell cultures from different precursors have allowed the production and study of megakaryocytes as well as proplatelets and platelets. However, the in vitro production of megakaryocytes
that culminate all stages of differentiation is a challenge that has not yet been resolved. In this work we report the
findings related to the determination of thrombopoietin treatment conditions and concentrations to achieve an
optimal relationship between the amount of thrombopoietin and the percentage and degree of megakaryocytic
differentiation in five allogeneic donors that were accepted for bone marrow transplantation.
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Copyright (c) 2021 Gerardo Romanelli, Silvia Olivera-Bravo, Carolina Beloso, Lucía Veiga, Noelia García, Hugo Giordano, Juan Claudio Benech, Inés Rauschert, Gustavo Folle, Adriana Mimbacas
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