Protection of chlorogenic acid against benzo[a]pyrene-induced lesions in Saccharomyces cerevisiae
Abstract
The aim of this study was to analyze the effect of chlorogenic acid, a polyphenolic compound found at high
concentrations in Ilex paraguariensis infusions, on cellular and molecular damage induced by benzo[a]pyrene.
Ilex paraguariensis infusions (“mate”) are consumed by most of the population in Argentina, Paraguay, southern Brazil and Uruguay. Saccharomyces cerevisiae yeast (SC7K lys2-3; SX46A and SX46A rad14Δ strains) were used as eukaryotic model organisms. Cells in an exponential growth phase were exposed to increasing
concentrations of benzo[a]pyrene, as well as combined treatments of benzo[a]pyrene at a concentration of
250 ng/mL and chlorogenic acid at a concentration matching that which is commonly found in mate.
Determinations of surviving fraction, mutagenic frequency and double strand DNA breaks induction were
performed, along with the assessment of the modulation of the expression of protein Rad14 by Western Blot.
A significant increase of surviving fractions and a decrease in mutagenic frequency were observed after
exposure to benzo[a]pyrene plus chlorogenic acid, contrary to benzo[a]pyrene alone. A substantial increase in sensitivity to benzo[a]pyrene was observed for the Rad14 protein-deficient mutating strain when compared to the SC7K lys2-3 strain. An important decrease in lethality was observed when combined benzo[a]pyrene and chlorogenic acid treatments were applied. As for the determination of DSBs, no chromosomic fractionation was observed at the benzo[a]pyrene concentration tested in the experiments. Western Blot analysis showed an increase in the expression of protein Rad14 for samples treated with benzo[a]pyrene as a single agent when compared against the control sample. Additionally, the expression of this protein was observed to diminish when combined treatments with benzo[a]pyrene and chlorogenic acid were used. Results obtained indicate that chlorogenic acid significantly decreases the mutagenic activity of benzo[a]pyrene, which is not related to an increase in the removal of lesions induced by nucleotide excision repair system.
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Copyright (c) 2023 Verónica Sosa, Lourdes Blanc, Eduardo Dellacassa, Nelson Bracesco
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